Journal:

Article Title: Effects of Environmental Enrichment on Spatial Memory and Neurochemistry in Middle-Aged Mice

doi: 10.1101/lm.50703

Figure Lengend Snippet: GAD and Synaptophysin Data for Each Group

Article Snippet: A total of 50 μL of 0.75 μg/mL monoclonal mouse anti-synaptophysin antibody (Clone SY 38, DAKO Corp.) in block buffer was then incubated in each well overnight at 4°C.

Techniques:

Journal: Scientific Reports

Article Title: Spatial training preserves associative memory capacity with augmentation of dendrite ramification and spine generation in Tg2576 mice

doi: 10.1038/srep09488

Figure Lengend Snippet: Antibodies employed in the study

Article Snippet: Syp , Total synaptophysin , mAb , 1:1000 , , Cell Signaling.

Techniques: Membrane

Journal: International Journal of Molecular Sciences

Article Title: Combination BMSC and Niaspan Treatment of Stroke Enhances White Matter Remodeling and Synaptic Protein Expression in Diabetic Rats

doi: 10.3390/ijms141122221

Figure Lengend Snippet: Treatment of stroke with BMSCs, Niaspan and combination BMSCs+Niaspan all significantly increased SMI-31 ( A , p < 0.05) expression compared to T1DM-MCAo control. Niaspan and combination BMSCs+Niaspan treatment both increased Synaptophysin ( B , p < 0.05) expression in the ischemic brain compared to T1DM-MCAo control. Combination treatment additively increases Synaptophysin expression compared to BMSCs monotherapy rats ( p < 0.05). Group number in T1DM-MCAo: n = 8; Niaspan-alone: n = 10; BMSCs-alone: n = 8; BMSCs + Niaspan: n = 7. * p < 0.05 vs. T1DM-MCAo control.

Article Snippet: After blocking in normal serum, brain sections were treated with the primary antibodies against NG2 (oligodendrocyte progenitor cell marker, 1:400; Millipore, Billerica, MA, USA), CNPase (a prenylated myelin protein, 1:200; Millipore, Billerica, MA, USA), Synaptophysin (monoclonal antibody; dilution 1:500, Millipore, MA, USA, and SMI-31 (a pan-axonal neurofilament marker, Neurofilaments, phosphorylated monoclonal antibody, 1:1000, Covance, Emeryville, CA, USA) overnight.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Combination BMSC and Niaspan Treatment of Stroke Enhances White Matter Remodeling and Synaptic Protein Expression in Diabetic Rats

doi: 10.3390/ijms141122221

Figure Lengend Snippet: Western Blot assay. ( A ) Western blot assay for NG2, CNPase, Synaptophysin and SMI-31; ( B – E ) Western blot quantitative data for NG2 ( B ); CNPase ( C ); Synaptophysin ( D ) and SMI-31 ( E ). n = 4/group.

Article Snippet: After blocking in normal serum, brain sections were treated with the primary antibodies against NG2 (oligodendrocyte progenitor cell marker, 1:400; Millipore, Billerica, MA, USA), CNPase (a prenylated myelin protein, 1:200; Millipore, Billerica, MA, USA), Synaptophysin (monoclonal antibody; dilution 1:500, Millipore, MA, USA, and SMI-31 (a pan-axonal neurofilament marker, Neurofilaments, phosphorylated monoclonal antibody, 1:1000, Covance, Emeryville, CA, USA) overnight.

Techniques: Western Blot